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Several factors contribute to the acquisition by retinal microglia of a pro-inflammatory phenotype during ageing, including altered metabolism and reduced immunomodulatory signals from retinal neurons, such as the CX3CL1-CX3CR1 and CD200-CD200R signaling pathways, resulting in increased microglial activation ( As with microglial cells, astrocyte activation is also one of the hallmarks of physiological ageing (Nichols et al., 1993

Moreover, the higher reproducibility of DIA compared to DDA likely comes from the multiple fragments measured over an elution profile, whereas single elution profile quantification is performed in DDA

Cell 75 , 487493 (1993)