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Analysis of pooled data To increase the sample size and BW range, five independent experiments totalling 145 rats were pooled into vehicle (V, n = 38), drug (CS + C + S, n = 57) and calorie restriction (WM + PF, n = 50) groups (Extended Data Table 1)

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Fluorescence images of fluorophores were captured on the basis of the preset wavelength settings for DAPI (for DAPI staining), Red (for Alexa Fluor 594) and Green (for Alexa Fluor 488) on the IN Cell microscope

At a mid-July rally where the Flint Journal estimated that about 100 people protested the rates, Councilman Wantwaz Davis said, Were going to keep going until we get national attention