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FIGURE 2 In 1976 Benson and Talalay discovered that the cytosolic fraction of rat liver catalyzes the same steroid isomerization of 5-androsten-3,17-dione, but in distinction from the bacterial enzyme the reaction included reduced glutathione as a designated coenzyme ( 1 mg 1 ) in comparison with the five orders of magnitude higher activity of the Pseudomonas enzyme ( In retrospect, it is clear that the ketosteroid isomerase activity of the GST in human liver ( 1 B 1 and B 1 B 2 in Stockman et al., 1985)

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Cells were then fixed and permeabilized for 20 min on ice using 100 l 1X CytoFix solution (BD Bioscience Cat