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In our Affinity GST Purification Protocol, we recommend using 1x PBS, pH 7.3 as the binding buffer

They do this using a special number called a CPT code

The induced toxic BA tolerance together with (i) increased LRO traffic/deposition of metabolites (ii) increased expression of LRO-specific mRNAs including biogenesis genes and membrane transporters and (iii) enhanced LRO Nile Red staining (Figs