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In support of this increased NK cytotoxic potential, the expression of the NK cytotoxic effector-associated gene perforin, as determined by flow-cytometric analysis or quantitative real-time PCR analysis in CD56 + NK cells, was also significantly increased in the above IGF-1-induced CD34 + -derived NK cells compared with the cells without IGF-1 (Fig

Metabolic activity was determined using a WST1 assay (Cayman) according to the manufactures protocol, after exposure to increasing concentrations of cuprizone (0250 M) for up to 72 h, or 20 M peroxide as positive control

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