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Briefly, cells fixed with 4% paraformaldehyde were blocked with 10% goat serum and 0.1% Triton-X in phosphate-buffered saline (PBS) at room temperature for 45 min, and thereafter incubated with primary antibody overnight at 4 C

randomized, and balanced samples (12) with 1 spiked QC sample analysed every 4 samples

Pros: Potent dosage Includes alpha-lipoic acid Good value for money Cons: Contains artificial sweeteners Some users report inconsistent results 6

Xiaoyaosan improves antibiotic-induced depressive-like and anxiety-like behavior in mice through modulating the gut microbiota and regulating the NLRP3 inflammasome in the colon