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After treatment, the supernatant and cells were collected, washed with ice-cold 1X PBS, and stained using a dual staining method with Annexin V-FITC and propidium iodide (PI) (BioLegend, San Diego, CA, USA), according to the manufacturer's instructions

AAPS PharmSciTech 24(2):66 Jumelle C et al (2020) Advances and limitations of drug delivery systems formulated as eye drops

With 16.67% niacinamide, glutathione, and RH-oligopeptide-1, this formula delivers visible brightening while calming and strengthening the skin

These findings suggest that these bioactive metabolites hold promise for development into therapeutic agents for neurodegenerative disorders, including Alzheimers disease