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Vacuolar localization of secondary metabolites has been suggested long time ago but it was experimentally demonstrated using water-free environment gradient centrifugation of Arabidopsis leaf tissue only recently ( Figure 2 , transporters 10 and 11) and we consider them below

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The results indicated a reduction in levels of ROS, MDA, and iron in the blood, an increase in the expression of GSH and GPX4, and alleviation of oxidative stress-induced mitochondrial damage in the spinal cord