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Because this step is so decisive, it has its own guide

A control assay may be performed in the absence of the compound to be screened and compared to the amount of fibroblast proliferation in the presence of the compound to determine if the compound stimulates proliferation by determining the uptake of 3[H] thymidine in each case

Nsiah-Dosu S, Scholz C, Orinska Z, Sadik CD, Ludwig RJ, Schmidt E, et al

In colon cancer HCT116 cells, knockdown of CD44 with small interference RNA substantially hampered cell proliferation, migration and invasion and enhanced cell apoptosis, accompanied by suppression of the phosphorylation of Akt and glycogen synthase kinase-3 (GSK-3), a decrease in the expression of Bcl-2 and Bcl-xl, and upregulation of caspase-3 and caspase-9 [117]