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Testosterone has a twofold lower affinity than 5-dihydrotestosterone for the androgen receptor, while the dissociation rate of testosterone from the receptor is five-fold faster than of 5-dihydrotestosterone (5)

To eliminate the risk of evaluating contaminating microorganisms from the environment in downstream analysis, negative (sample-free) controls were processed simultaneously with the samples of the experiment

Pathways affecting lipid metabolism and cell junctions, such as mitogen-activated protein kinase (MAPK) signaling, peroxisome proliferator-activated receptor (PPAR) signaling, tight junction, extracellular matrix (ECM)receptor interactions, focal adhesion, and regulation of the actin cytoskeleton, were suggested to potentially contribute to IMF deposition [29]

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