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Arch Biochem Biophys 487(2):146152 Lee DH, Silventoinen K, Jacobs DR Jr, Jousilahti P, Tuomileto J (2004) Gamma-glutamyltransferase, obesity, and the risk of type 2 diabetes: observational cohort study among 20,158 middle-aged men and women

After spinning, supernatant was removed and pellet was resuspended in 0.1 M sodium cacodylate buffer (pH 7,4) + 2.5% glutaraldehyde and stored overnight at 4 C

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By inhibiting the activity of Tyrosinase enzyme, making the skin white by inhibiting the process of melanin production