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Then 3,4-dimethoxyphenethylamine was added (1.25 mmol) and the solution cooled to 0C before dichloromethane solution containing 4-DMAP (40 mg) was added dropwise to the reaction system, which was maintained at 0C for 1 hour

Veng-Pedersen P, Paustenbach DJ, Carlson GP, & Suarez L (1987) A linear systems approach to analyzing the pharmacokinetics of carbon tetrachloride in the rat following repeated exposures of 8 and 11.5 h/day

Interestingly, lipid peroxidation products including 4-hydroxynonenal have been shown to modify TOP1 active site residue C630 leading to TOP1 entrapment on DNA (117)

Then, it was reverse transcribed using SuperScript Vilo cDNA Synthesis kit