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This fragmentation creates coordination failures: Regulatory gaps: Smoked fish contaminated with PAHs violates food safety standards, but enforcement mechanisms focus on microbial contamination and acute chemical hazards (pesticide residues, aflatoxins) rather than thermal processing contaminants requiring specialized analytical capacity

Among the possible options for the substrate binding to the active site of the enzyme under study, only the mechanism involving primary ATP binding allows obtaining a value for the reaction rate corresponding to the experimentally measured glutamate-cysteine ligase activity at physiological substrate levels

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