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In addition, IHC analysis revealed that the P-gp, LC3B, VDAC1, and Ki-67 levels were highest in the HGC-27R-EVs-shNC and HGC-27R-EVs-shNC/PTX groups with no significant difference, suggesting that the transmission of PTX resistance by EV-derived VDAC1 protein through autophagy led to a reduction in the sensitivity of GC cells to PTX ( Figures 6D and S3C )

Fasting a little bit is good, fasting for three days is even better at reversing misfolded proteins and inducing chaperone-mediated autophagy [cellular regeneration], which helps maintain cellular proteostasis. 5

Cellular metabonomic analysis by 1 H-NMR Freeze-dried intracellular metabolites extracts were dissolved in 600 l phosphate buffer 0.1 m (pH 7.4, 99.9% D 2 O) containing 0.001% sodium 3-trimethylsilyl-1-[2,2,3,3- 2 H4] propionate as previously described [48]